Sequence of EMB-1, an mRNA accumulating specifically in embryos of carrot.

We have screened a lambda gtlO cDNA library synthesized from poly A RNA isolated from carrot somatic embryos to identify genes whose expression was associated with embryogenesis. 200,000 recombinant phage were differentially hyridized with single-stranded, 32P-labelled cDNA synthesized from poly A RNA isoalted from callus cells (1) and from embryos (2) of Daucus carota. This screen identified EMB-1, an mRNA that uniquely accumulates in embryos. The derived amino acid sequence of this clone shows similarity to the Em protein of wheat (3) and the D19 mRNA and protein of cotton (4). These proteins are translated from Lea mRNAs, which accumulate to high levels in very late staged zygotic embryos of wheat and cotton, respectively (3, 5). This is the first report of a Lea protein of group 1(4) accumulating in somatic embryos. Disciplines Biochemistry, Biophysics, and Structural Biology | Botany | Food Science Comments This is an article from Nucleic Acids Research 18 (1990): 2826. Posted with permission. Rights This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This article is available at Iowa State University Digital Repository: http://lib.dr.iastate.edu/bbmb_ag_pubs/59 2826 Nucleic Acids Research, Vol. 18, No. 9 Sequence of EMB-1, an mRNA accumulating specifically in embryos of carrot T.U.Ulrich4, E.S.Wurtele1,3*, B.J.Nikolau2'3 1Department of Botany, 2Department of Biochemistry and Biophysics, 3Department of Food Technology and Center for Crops Utilization Research, Iowa State University, Ames, IA 50011 and 4Sogetol, Inc., 3876 Bay Place Center, Hayward, CA 94545, USA Submitted December 18, 1989 We have screened a lambda gtlO cDNA library synthesized from poly A RNA isolated from carrot somatic embryos to identify genes whose expression was associated with embryogenesis. 200,000 recombinant phage were differentially hyridized with single-stranded, 32P-labelled cDNA synthesized from poly A RNA isoalted from callus cells (1) and from embryos (2) of Daucus carota. This screen identified EMB-1, an mRNA that uniquely accumulates in embryos. The derived amino acid sequence of this clone shows similarity to the Em protein of wheat (3) and the D19 mRNA and protein of cotton (4). These proteins are translated from Lea mRNAs, which accumulate to high levels in very late staged zygotic embryos of wheat and cotton, EMBL accession no. X17608 respectively (3, 5). This is the first report of a Lea protein of group 1(4) accumulating in somatic embryos.